The RpoN-box motif of the RNA polymerase sigma factor sigma(N) plays a role in promoter recognition

被引：43

作者：

Taylor, M ^{[1
]}

Butler, R ^{[1
]}

Chambers, S ^{[1
]}

Casimiro, M ^{[1
]}

Badii, F ^{[1
]}

Merrick, M ^{[1
]}

机构：

[1] JOHN INNES CTR PLANT SCI RES,NITROGEN FIXAT LAB,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1996年 / 22卷 / 05期

关键词：

D O I：

10.1046/j.1365-2958.1996.01547.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNA polymerase sigma factor sigma(N) (sigma(54)) is characterized by the presence, near the C-terminal end of the protein, of a highly conserved sequence of 10 amino acids (ARRTVAKYRE) that has been termed the RpoN box. In order to examine the function of this motif, which is predicted to adopt an alpha-helical stucture, we have isolated a number of mutations that alter residues within the box and examined the properties of the sigma(N) derivatives encoded by them. Certain mutations that alter charged and potentially exposed residues within the motif result in transcriptionally inactive proteins with impaired promoter recognition but no impairment in core RNA polymerase binding. We therefore suggest that the RpoN box could play a direct or indirect role in recognition of the -24, -12 promoter consensus that is characteristic of sigma(N)-dependent genes.

引用

页码：1045 / 1054

页数：10

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