Evidence for a SAL1-PAP Chloroplast Retrograde Pathway That Functions in Drought and High Light Signaling in Arabidopsis

被引:415
作者
Estavillo, Gonzalo M. [1 ]
Crisp, Peter A. [1 ]
Pornsiriwong, Wannarat [1 ]
Wirtz, Markus [2 ]
Collinge, Derek [1 ]
Carrie, Chris [3 ]
Giraud, Estelle [3 ]
Whelan, James [3 ]
David, Pascale [4 ]
Javot, Helene [4 ]
Brearley, Charles [5 ]
Hell, Ruediger [2 ]
Marin, Elena [4 ]
Pogson, Barry J. [1 ]
机构
[1] Australian Natl Univ Canberra, Res Sch Biol, Australian Res Council Ctr Excellence Plant Energ, Acton, ACT 0200, Australia
[2] Heidelberg Univ, Heidelberg Inst Plant Sci, D-69120 Heidelberg, Germany
[3] Univ Western Australia, ARC Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia
[4] CEA, Direct Sci Vivant Inst Biol Environm & Biotechnol, Unite Mixte Rech 6191, CNRS,Lab Biol Dev Plantes, F-13108 St Paul Les Durance, France
[5] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
基金
澳大利亚研究理事会;
关键词
MG-PROTOPORPHYRIN IX; INOSITOL POLYPHOSPHATE 1-PHOSPHATASE; PROMOTES TRANSCRIPTION TERMINATION; GENE-EXPRESSION; ABSCISIC-ACID; 3(2); 5-BISPHOSPHATE NUCLEOTIDASE; NEGATIVE REGULATOR; HYDROGEN-PEROXIDE; STRESS; RNA;
D O I
10.1105/tpc.111.091033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Compartmentation of the eukaryotic cell requires a complex set of subcellular messages, including multiple retrograde signals from the chloroplast and mitochondria to the nucleus, to regulate gene expression. Here, we propose that one such signal is a phosphonucleotide (3'-phosphoadenosine 5'-phosphate [PAP]), which accumulates in Arabidopsis thaliana in response to drought and high light (HL) stress and that the enzyme SAL1 regulates its levels by dephosphorylating PAP to AMP. SAL1 accumulates in chloroplasts and mitochondria but not in the cytosol. sal1 mutants accumulate 20-fold more PAP without a marked change in inositol phosphate levels, demonstrating that PAP is a primary in vivo substrate. Significantly, transgenic targeting of SAL1 to either the nucleus or chloroplast of sal1 mutants lowers the total PAP levels and expression of the HL-inducible ASCORBATE PEROXIDASE2 gene. This indicates that PAP must be able to move between cellular compartments. The mode of action for PAP could be inhibition of 5' to 3' exoribonucleases (XRNs), as SAL1 and the nuclear XRNs modulate the expression of a similar subset of HL and drought-inducible genes, sal1 mutants accumulate XRN substrates, and PAP can inhibit yeast (Saccharomyces cerevisiae) XRNs. We propose a SAL1-PAP retrograde pathway that can alter nuclear gene expression during HL and drought stress.
引用
收藏
页码:3992 / 4012
页数:21
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