Novel deposition of nano-sized silicon substituted hydroxyapatite by electrostatic spraying

被引:50
作者
Huang, J
Jayasinghe, S
Best, SM
Edirisinghe, MJ
Brooks, RA
Rushton, N
Bonfield, W
机构
[1] Univ Cambridge, Dept Mat Sci & Met, Cambridge CB2 3QZ, England
[2] Univ London, Queen Mary, Dept Mat, London E1 4NS, England
[3] Addenbrookes Hosp, Orthopaed Res Unit, Cambridge CB2 2QQ, England
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1007/s10856-005-4720-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Suspensions containing nano-sized silicon substituted hydroxyaptite (nSiHA) particles were produced and processed for electrostatic spray deposition. No secondary phases were detected by X-ray diffraction, which indicated that the nSiHA was phase pure. Electrostatic spraying of nSiHA in cone-jet mode was achieved at flow rate of 10(-9) m(3)s(-1) with an applied voltage between the needle and the ring-shaped ground electrode set at 6 to 8 kV. Micrometer- and submicrometer-scaled islands of nSiHA have been deposited on glass and titanium substrates. The surface roughness of such nHA and nSiHA islands was in the range 60 to 80 nm, as measured from atomic force microscopy in tapping mode. The growth of primary human osteoblast (HOB) cells on the nSiHA deposited substrates increased with time during the 4 days of culture, and the increase was related with the Si content in substituted HA, indicating that nSiHA was able to promote and support the growth of HOB cells. Scanning electron microscopy (SEM) revealed that extracellular matrix (ECM) produced by the HOB cells on these nSiHA deposits was well organized. In addition, the presence of Ca and P containing nodules in the ECM were also confirmed by Energy Dispersive X-ray (EDX) analysis, indicating early signs of calcification fronts. The results showed that nSiHA produced by electrostatic spray deposition was able to promote the attachment and the growth of HOB cells. Therefore, electrostatic spray deposition offers great potential for the creation of bioactive surfaces to provide improved interfacial bonding with host tissues. (C) 2005 Springer Science + Business Media, Inc.
引用
收藏
页码:1137 / 1142
页数:6
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