Use of the Gal4-UAS technique for targeted gene expression in the zebrafish

被引：314

作者：

Scheer, N ^{[1
]}

Campos-Ortega, JA ^{[1
]}

机构：

[1] Univ Cologne, Inst Entwicklungsbiol, D-50923 Cologne, Germany

来源：

MECHANISMS OF DEVELOPMENT | 1999年 / 80卷 / 02期

关键词：

zebrafish; GAL4-UAS system; transgenic lines;

D O I：

10.1016/S0925-4773(98)00209-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The most common way to analyze the function of cloned genes in zebrafish is to misexpress the gene product or an altered variant of it by mRNA injection. However, mRNA injection has several disadvantages. The GAL4-UAS system for targeted gene expression allows one to overcome some of these disadvantages. To test the GAIA-UAS system in zebrafish, we generated two different kinds of stable transgenic lines, carrying activator and effector constructs, respectively. In the activator Lines the gene for the yeast transcriptional activator GAL4 is under the control of a given promoter, while in the effecters the gene of interest is fused to the sequence of the DNA-binding motif of GAL4 (UAS). Crosses of animals from the activator and effector lines show that effector genes are transcribed with the spatial pattern of the activators. This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：153 / 158

页数：6

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