A Pseudomonas aeruginosa biosensor responds to exposure to ultraviolet radiation

被引：20

作者：

Elasri, MO ^{[1
]}

Miller, RV ^{[1
]}

机构：

[1] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1998年 / 50卷 / 04期

关键词：

D O I：

10.1007/s002530051320

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisheri lux operon. This recA-lux fusion (pMOE15) was introduced into wild-type P. aeruginosa strain FRD1 and recA expression was monitored by measuring 490-nm light production. The RM4440 strain responded to increasing doses of ultraviolet radiation by an increase in its bioluminescence. RM4440 has the potential to be useful as a biosensor for the presence of DNA-damaging agents in the environment.

引用

页码：455 / 458

页数：4

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