Lipid phosphate phosphatase-1 dephosphorylates exogenous lysophosphatidate and thereby attenuates its effects on cell signalling

被引：17

作者：

Pilquil, C ^{[1
]}

Singh, I ^{[1
]}

Zhang, QX ^{[1
]}

Ling, ZC ^{[1
]}

Buri, K ^{[1
]}

Stromberg, LM ^{[1
]}

Dewald, J ^{[1
]}

Brindley, DN ^{[1
]}

机构：

[1] Univ Alberta, Dept Biochem, Signal Transduct Res Grp, Edmonton, AB T6G 2S2, Canada

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2001年 / 64卷 / 1-4期

关键词：

cAMP; cell division; EDG; receptors; mitogen-activated; protein kinase; phosphatidate; phosphohydrolase; phospholipase;

D O I：

10.1016/S0090-6980(01)00101-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The serum-derived phospholipid growth factor, lysophosphatidate (LPA), activates cells through a family of G-protein-coupled EDG receptors. The present article examines the role of lipid phosphate phosphatase-1 (LPP-1, or phosphatidate phosphate 2A) in regulating cell activation by LPA. Over-expressing LPP-1 approximately doubled the rate of dephosphorylation of exogenous LPA by Rat2 fibroblasts. The amount of LPA dephosphorylation was restricted to less than 10% of the total exogenous LPA. Over-expression of LPP-1 attenuated cell activation as indicated by diminished responses including cAMP, Ca2+, activation of phospholipase D and ERK, DNA synthesis and cell division. LPP-1 therefore provides a novel level of regulation for controlling cell signalling by exogenous LPA. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：83 / 92

页数：10

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