Genomic organization and transcriptional regulation of the mouse GD3 synthase gene (ST8Sia I): Comparison of genomic organization of the mouse sialyltransferase genes

被引:19
作者
Takashima, S [1 ]
Kono, M
Kurosawa, N
Yoshida, Y
Tachida, Y
Inoue, M
Kanematsu, T
Tsuji, S
机构
[1] RIKEN, Inst Phys & Chem Res, Frontier Res Program, Wako, Saitama 3510198, Japan
[2] Biooriented Technol Res Advancement Inst, Minato Ku, Tokyo 1050001, Japan
关键词
GD3; synthase; genomic organization; molecular cloning; sialyltransferase; transcriptional regulation;
D O I
10.1093/oxfordjournals.jbchem.a022831
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genomic organization of the gene encoding the mouse GD3 synthase (ST8Sia I) has been determined. The mouse ST8Sia I gene spans over 100 kilobases of genomic DNA with a unique genomic structure of 5 exons, Analysis of the sequence immediately upstream of the transcription initiation site revealed that the ST8Sia I promoter contained no canonical TATA- or CCAAT-box, but contained a putative Spl binding site. Transient transfection experiments demonstrated functional promoter activity of the ST8Sia I promoter in an ST8Sia I-expressing cell line, P19, but not in an ST8Sia I-nonexpressing cell line, NIH3T3, Mobility shift assay and mutation analysis of the promoter region indicated that the Spl binding site is involved in the transcriptional regulation of the ST8Sia I gene in pie cells. Here, the genomic structural analyses of mouse sialyltransferase genes are summarized and the genomic structures of these genes are compared.
引用
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页码:1033 / 1043
页数:11
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