Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

被引:162
作者
Ki, Chang Seok [1 ,2 ]
Park, Sook Young [1 ,2 ,3 ,4 ]
Kim, Hyun Jeong [2 ,3 ,4 ]
Jung, Hong-Moon [5 ]
Woo, Kyung Mi [5 ]
Lee, Jung Weon [6 ]
Park, Young Hwan [2 ]
机构
[1] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South Korea
[2] Seoul Natl Univ, Intelligent Text Res Ctr, Seoul 151742, South Korea
[3] Seoul Natl Univ, Dent Res Inst, Seoul 110768, South Korea
[4] Seoul Natl Univ, Dept Dent Anesthesiol, Seoul 110768, South Korea
[5] Seoul Natl Univ, Sch Dent, Dept Cell & Dev Biol, Seoul 110768, South Korea
[6] Seoul Natl Univ, Coll Med, Inst Canc Res, Dept Tumor Biol, Seoul 110799, South Korea
关键词
biomaterials; cell adhesion; cell proliferation; fibroins; osteoblasts;
D O I
10.1007/s10529-007-9581-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
引用
收藏
页码:405 / 410
页数:6
相关论文
共 11 条
[1]
Silk-based biomaterials [J].
Altman, GH ;
Diaz, F ;
Jakuba, C ;
Calabro, T ;
Horan, RL ;
Chen, JS ;
Lu, H ;
Richmond, J ;
Kaplan, DL .
BIOMATERIALS, 2003, 24 (03) :401-416
[2]
Tyrosine phosphorylation of paxillin affects the metastatic potential of human osteosarcoma [J].
Azuma, K ;
Tanaka, M ;
Uekita, T ;
Inoue, S ;
Yokota, J ;
Ouchi, Y ;
Sakai, R .
ONCOGENE, 2005, 24 (30) :4754-4764
[3]
Mechanisms of focal adhesion kinase regulation [J].
Cohen, LA ;
Guan, JL .
CURRENT CANCER DRUG TARGETS, 2005, 5 (08) :629-643
[4]
Integrins and the actin cytoskeleton [J].
Delon, Isabelle ;
Brown, Nicholas H. .
CURRENT OPINION IN CELL BIOLOGY, 2007, 19 (01) :43-50
[5]
Integrin signaling to the actin cytoskeleton [J].
DeMali, KA ;
Wennerberg, K ;
Burridge, K .
CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (05) :572-582
[6]
Domain structure and organisation in extracellular matrix proteins [J].
Hohenester, E ;
Engel, J .
MATRIX BIOLOGY, 2002, 21 (02) :115-128
[7]
Regulation of G1 phase progression by growth factors and the extracellular matrix [J].
Hulleman, E ;
Boonstra, J .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (01) :80-93
[8]
Lee JW, 2004, MOL CELLS, V17, P188
[9]
Polymeric scaffolds for bone tissue engineering [J].
Liu, XH ;
Ma, PX .
ANNALS OF BIOMEDICAL ENGINEERING, 2004, 32 (03) :477-486
[10]
SCHLAEPFER DD, 1994, NATURE, V372, P786