Target-selected mutant screen by TILLING in Drosophila

被引:71
作者
Winkler, S
Schwabedissen, A
Backasch, D
Bökel, C
Seidel, C
Bönisch, S
Fürthauer, M
Kuhrs, A
Cobreros, L
Brand, M
González-Gaitán, M
机构
[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[2] Dresden Univ Technol, D-01062 Dresden, Germany
关键词
D O I
10.1101/gr.3721805
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The availability of the full Drosophila genomic DNA sequence prompts the development of a method to efficiently obtain mutations in genes of interest identified by their sequence homologies or biochemically. To date, molecularly characterized Mutations have been generated in around 6000 of the -15,000 annotated fly genes, of which around one-third are essential for viability. To obtain Mutations in essential and nonessential genes of interest, we took a reverse genetics approach, based oil the large-scale detection Of point Mutations by Cel-I-mediated heteroduplex cleavage. A library of genomic DNA from 2086 EMS-mutagenized lines was established. The library was screened for mutations ill three genes. A total of 6.1 Mb were screened, and 44 hits were found in two different mutagenesis conditions. Optimal conditions yielded ail average of one Mutation every 156 kb. For ail essential gene tested, five of 25 Mutations turned Out to cause lethality, confirming that EMS mutagenesis leads to high frequency of gene inactivation. We thereby established that Cel-I-mediated TILLING call be Used to efficiently obtain mutations in genes of interest in Drosophila.
引用
收藏
页码:718 / 723
页数:6
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