Zika NS1-induced ER remodeling is essential for viral replication

被引：65

作者：

Ci, Yali ^{[1
,2
,3
]}

Liu, Zhong-Yu ^{[4
,5
]}

Zhang, Na-Na ^{[5
]}

Niu, Yuqiang ^{[6
]}

Yang, Yang ^{[1
,2
,3
]}

Xu, Caimin ^{[1
,2
,3
]}

Yang, Wei ^{[6
]}

Qin, Cheng-Feng ^{[5
,7
]}

Shi, Lei ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing, Peoples R China

[2] Peking Union Med Coll, Sch Basic Med, Beijing, Peoples R China

[3] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biochem & Mol Biol, Beijing, Peoples R China

[4] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Guangzhou, Peoples R China

[5] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China

[6] Chinese Acad Med Sci, Inst Pathogen Biol, Beijing, Peoples R China

[7] First Hosp Jilin Univ, Changchun, Peoples R China

来源：

JOURNAL OF CELL BIOLOGY | 2020年 / 219卷 / 02期

基金：

中国国家自然科学基金;

关键词：

NONSTRUCTURAL PROTEINS NS1; TERMINAL AMPHIPATHIC HELIX; DENGUE VIRUS; MEMBRANE ASSOCIATION; ACTIVATION; SYNAPTOTAGMIN; ORGANELLES; CURVATURE; MUTATION; NS4A;

D O I：

10.1083/jcb.201903062

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Zika virus (ZIKV), a recently emerged member of the flavivirus family, forms replication compartments at the ER during its lifecycle. The proteins that are responsible for the biogenesis of replication compartments are not well defined. Here, we show that Zika nonstructural protein 1 (NS1)-induced ER remodeling is essential for viral replication. NS1 expressed in the ER lumen induced ER perinuclear aggregation with an ultrastructure resembling that of the replication compartment. Data from model membrane system indicated that the membrane-binding and membrane-remodeling properties of NS1 depend on its hydrophobic insertion into the membrane. These findings demonstrate that NS1 plays a crucial role in flavivirus replication compartment formation by remodeling the ER structure.

引用

页数：13

共 50 条

[1]

Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System [J].

Akey, David L. ;

Brown, W. Clay ;

Dutta, Somnath ;

Konwerski, Jamie ;

Jose, Joyce ;

Jurkiw, Thomas J. ;

DelProposto, James ;

Ogata, Craig M. ;

Skiniotis, Georgios ;

Kuhn, Richard J. ;

Smith, Janet L. .

SCIENCE, 2014, 343 (6173) :881-885

[2]

The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling [J].

Aktepe, Turgut E. ;

Liebscher, Susann ;

Prier, Julia E. ;

Simmons, Cameron P. ;

Mackenzie, Jason M. .

CELL REPORTS, 2017, 21 (06) :1639-1654

[3]

Coupling of replication and assembly in flaviviruses [J].

Apte-Sengupta, Swapna ;

Sirohi, Devika ;

Kuhn, Richard J. .

CURRENT OPINION IN VIROLOGY, 2014, 9 :134-142

[4]

Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication [J].

Belov, George A. ;

Habbersett, Courtney ;

Franco, David ;

Ehrenfeld, Ellie .

JOURNAL OF VIROLOGY, 2007, 81 (17) :9259-9267

[5]

Filter extrusion of liposomes using different devices: comparison of liposome size, encapsulation efficiency, and process characteristics [J].

Berger, N ;

Sachse, A ;

Bender, J ;

Schubert, R ;

Brandl, M .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 223 (1-2) :55-68

[6]

Extended surface for membrane association in Zika virus NS1 structure [J].

Brown, W. Clay ;

Akey, David L. ;

Konwerski, Jamie R. ;

Tarrasch, Jeffrey T. ;

Skiniotis, Georgios ;

Kuhn, Richard J. ;

Smith, Janet L. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016, 23 (09) :865-867

[7]

FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATION [J].

CHAMBERS, TJ ;

HAHN, CS ;

GALLER, R ;

RICE, CM .

ANNUAL REVIEW OF MICROBIOLOGY, 1990, 44 :649-688

[8]

Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection [J].

Chen, Jincheng ;

Ng, Mary Mah-Lee ;

Chu, Justin Jang Hann .

PLOS PATHOGENS, 2015, 11 (07)

[9]

MEMBRANE REARRANGEMENT AND VESICLE INDUCTION BY RECOMBINANT POLIOVIRUS 2C AND 2BC IN HUMAN-CELLS [J].

CHO, MW ;

TETERINA, N ;

EGGER, D ;

BIENZ, K ;

EHRENFELD, E .

VIROLOGY, 1994, 202 (01) :129-145

[10]

Ultrastructural Characterization of Zika Virus Replication Factories [J].

Cortese, Mirko ;

Goellner, Sarah ;

Acosta, Eliana Gisela ;

Neufeldt, Christopher John ;

Oleksiuk, Olga ;

Lampe, Marko ;

Haselmann, Uta ;

Funaya, Charlotta ;

Schieber, Nicole ;

Ronchi, Paolo ;

Schorb, Martin ;

Pruunsild, Priit ;

Schwab, Yannick ;

Chatel-Chaix, Laurent ;

Ruggieri, Alessia ;

Bartenschlager, Ralf .

CELL REPORTS, 2017, 18 (09) :2113-2123

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