Multifocal structure of the T cell - dendritic cell synapse

被引:155
作者
Brossard, C
Feuillet, V
Schmitt, A
Randriamampita, C
Romao, M
Raposo, G
Trautmann, A
机构
[1] Univ Paris 05, CNRS, INSERM, U567,UMR 8104,Dept Biol Cellulaire,Inst Cochin, F-75014 Paris, France
[2] Inst Curie, Res Sect, CNRS, UMR 144, Paris, France
关键词
antigen-presenting cells; dendritic cells; electron microscopy; LFA-1; T cells;
D O I
10.1002/eji.200425857
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free synapses present a number of tight appositions (cleft size similar to 15 nm), all along the synapse. These tight appositions cover a significantly larger surface fraction of antigen-dependent synapses. In immunofluorescence, antigen-dependent synapses show multiple patches of CD3 and LFA-1 with a variable overlap. A similar distribution is observed for PKC theta and talin. A concentric organization characteristic of prototypical synapses is rarely observed, even when dendritic cells are paralyzed by cytoskeletal poisons. In T-DC synapses, the interaction surface is composed of several tens of submicronic contact spots, with no large-scale segregation of CD3 and LFA-1. As a comparison, in T-B synapses, a central cluster of CD3 is frequently observed by immunofluorescence, and electron microscopy reveals a central tight apposition. Our data show that it is inappropriate to consider the concentric structure as a "mature synapse" and multifocal structures as immature.
引用
收藏
页码:1741 / 1753
页数:13
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