Type II phospholipase A(2) is linked to cyclooxygenase-2-mediated delayed prostaglandin D-2 generation by cultured mouse mast cells following Fc epsilon RI- and cytokine-dependent activation

被引：20

作者：

Ashraf, MDM ^{[1
]}

Murakami, M ^{[1
]}

Shimbara, S ^{[1
]}

Amakasu, Y ^{[1
]}

Atsumi, G ^{[1
]}

Kudo, I ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,DEPT HLTH CHEM,SHINAGAWA KU,TOKYO 142,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 229卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1872

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of murine bone marrow-derived mast cells (BMMC) with interleukin (IL)-10 and IL-1 beta increased the expression of type II secretory phospholipase A(2) (sPLA(2)), but not cytosolic PLA(2) (cPLA(2)), after culture for several hours. Further stimulation with IgE and antigen (IgE/Ag) increased cyclooxygenase (COX)-2 expression dramatically, accompanied by augmented delayed prostaglandin (PG) D-2 generation over several hours. BMMC were also found to express type IIC PLA(2) (PLA(2)-IIC), a recently described novel sPLA(2) possessing 16 cysteine residues, expression of which changed minimally after BMMC activation. Delayed PGD(2) generation was suppressed by similar to 80% by an antibody raised against recombinant murine type II sPLA(2). These results suggest that, of the three PLA(2)s expressed in BMMC, type II sPLA(2) is the critical enzyme that is coupled with COX-2-dependent PGD(2) generation elicited by IgE/Ag in the presence of IL-10 + IL-1 beta. (C) 1996 Academic Press

引用

页码：726 / 732

页数：7

共 28 条

[1]

ASHRAF M, 1996, IN PRESS BIOCH J

[2] Distinct roles in signal transduction for each of the phospholipase A(2) enzymes present in P388D(1) macrophages [J].

Balsinde, J ;

Dennis, EA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (12) :6758-6765

[3] A heparin-sensitive phospholipase A(2) and prostaglandin endoperoxide synthase-2 are functionally linked in the delayed phase of prostaglandin D-2 generation in mouse bone marrow-derived mast cells [J].

Bingham, CO ;

Murakami, M ;

Fujishima, H ;

Hunt, JE ;

Austen, KF ;

Arm, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (42) :25936-25944

[4]

CHEN J, 1994, J BIOL CHEM, V269, P23018

[5]

FONTEH AN, 1994, J IMMUNOL, V152, P5438

[6] TRANSLOCATION OF THE 85-KDA PHOSPHOLIPASE A(2) FROM CYTOSOL TO THE NUCLEAR-ENVELOPE IN RAT BASOPHILIC LEUKEMIA-CELLS STIMULATED WITH CALCIUM IONOPHORE OR IGE/ANTIGEN [J].

GLOVER, S ;

BAYBURT, T ;

JONAS, M ;

CHI, E ;

GELB, MH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) :15359-15367

[7] A REQUIREMENT FOR SYK IN THE ACTIVATION OF THE MICROTUBULE-ASSOCIATED PROTEIN-KINASE PHOSPHOLIPASE-A(2) PATHWAY BY FC-EPSILON-R1 IS NOT SHARED BY A G-PROTEIN-COUPLED RECEPTOR [J].

HIRASAWA, N ;

SCHARENBERG, A ;

YAMAMURA, H ;

BEAVEN, MA ;

KINET, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (18) :10960-10967

[8] PURIFICATION AND CHARACTERIZATION OF PHOSPHOLIPASE-A2 RELEASED FROM RAT PLATELETS [J].

HORIGOME, K ;

HAYAKAWA, M ;

INOUE, K ;

NOJIMA, S .

JOURNAL OF BIOCHEMISTRY, 1987, 101 (03) :625-631

[9]

LIN LL, 1992, J BIOL CHEM, V267, P23451

[10] THE SECRETORY PHOSPHOLIPASE-A2 GENE IS A CANDIDATE FOR THE MOM1 LOCUS, A MAJOR MODIFIER OF APC(MIN)-INDUCED INTESTINAL NEOPLASIA [J].

MACPHEE, M ;

CHEPENIK, KP ;

LIDDELL, RA ;

NELSON, KK ;

SIRACUSA, LD ;

BUCHBERG, AM .

CELL, 1995, 81 (06) :957-966

← 1 2 3 →