Phosphorylation of Ser 402 impedes phosphatase activity of slingshot 1

被引：21

作者：

Barisic, Sandra ^{[2
]}

Nagel, Anja C. ^{[1
]}

Franz-Wachtel, Mirita ^{[3
]}

Macek, Boris ^{[3
]}

Preiss, Anette ^{[1
]}

Link, Gisela ^{[2
]}

Maier, Dieter ^{[1
]}

Hausser, Angelika ^{[2
]}

机构：

[1] Univ Hohenheim, Inst Gen Genet, D-70599 Stuttgart, Germany

[2] Univ Stuttgart, Inst Cell Biol & Immunol, D-70569 Stuttgart, Germany

[3] Univ Tubingen, Proteome Ctr Tuebingen, Interfac Inst Cell Biol, D-72076 Tubingen, Germany

来源：

EMBO REPORTS | 2011年 / 12卷 / 06期

关键词：

cofilin; protein kinase D; slingshot; focal adhesion; PROTEIN-KINASE-D; ACTIN REORGANIZATION; REGULATES COFILIN; CELL-MIGRATION; LIM KINASE; FAMILY; SPECIFICITY; ACTIVATION; IDENTIFICATION; SUBSTRATE;

D O I：

10.1038/embor.2011.53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using mass spectrometry, we have identified Ser 402 as a new phosphorylation site within the catalytic domain of human slingshot 1 (SSH1). Phosphorylation at this site inhibits substrate binding and, thus, phosphatase activity in vitro, resulting in enrichment of phosphorylated cofilin in monolayer cell culture. We further demonstrate that protein kinase D (PKD) is upstream from Ser 402 phosphorylation. Accordingly, expression of active PKD in Drosophila phenotypically mimics the loss of SSH activity by inducing accumulation of phosphorylated cofilin and filamentous actin. We thus identify a universal mechanism by which PKD controls SSH1 phosphatase activity.

引用

页码：527 / 533

页数：7

共 19 条

[1] Cofilin takes the lead [J].

DesMarais, V ;

Ghosh, M ;

Eddy, R ;

Condeelis, J .

JOURNAL OF CELL SCIENCE, 2005, 118 (01) :19-26

[2] A phosphorylation state-specific antibody recognizes Hsp27, a novel substrate of protein kinase D [J].

Döppler, H ;

Storz, P ;

Li, J ;

Comb, MJ ;

Toker, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (15) :15013-15019

[3] Protein kinase D1 regulates cofilin-mediated F-actin reorganization and cell motility through slingshot [J].

Eiseler, Tim ;

Doeppler, Heike ;

Yan, Irene K. ;

Kitatani, Kanae ;

Mizuno, Kensaku ;

Storz, Peter .

NATURE CELL BIOLOGY, 2009, 11 (05) :545-U64

[4] Structural requirements for localization and activation of protein kinase Cμ (PKCμ) at the Golgi compartment [J].

Hausser, A ;

Link, G ;

Bamberg, L ;

Burzlaff, A ;

Lutz, S ;

Pfizenmaier, K ;

Johannes, FJ .

JOURNAL OF CELL BIOLOGY, 2002, 156 (01) :65-74

[5] A rapid method for determining protein kinase phosphorylation specificity [J].

Hutti, JE ;

Jarrell, ET ;

Chang, JD ;

Abbott, DW ;

Storz, P ;

Toker, A ;

Cantley, LC ;

Turk, BE .

NATURE METHODS, 2004, 1 (01) :27-29

[6] AN EMERGING FAMILY OF DUAL-SPECIFICITY MAP KINASE PHOSPHATASES [J].

KEYSE, SM .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1995, 1265 (2-3) :152-160

[7] The slingshot family of Phosphatases mediates rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility Behavior of Keratinocytes [J].

Kligys, Kristina ;

Claiborne, Jessica N. ;

Debiase, Phillip J. ;

Hopkinson, Susan B. ;

Wu, Yvonne ;

Mizuno, Kensaku ;

Jones, Jonathan C. R. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (44) :32520-32528

[8] Molecular Dissection of the Mechanisms of Substrate Recognition and F-actin-mediated Activation of Cofilin-phosphatase Slingshot-1 [J].

Kurita, Souichi ;

Watanabe, Yosuke ;

Gunji, Emi ;

Ohashi, Kazumasa ;

Mizuno, Kensaku .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (47) :32542-32552

[9] Protein Kinase D regulates several aspects of development in Drosophila melanogaster [J].

Maier, Dieter ;

Nagel, Anja C. ;

Gloc, Helena ;

Hausser, Angelika ;

Kugler, Sabrina J. ;

Wech, Irmgard ;

Preiss, Anette .

BMC DEVELOPMENTAL BIOLOGY, 2007, 7

[10] Coronin 2A regulates a subset of focal-adhesion-turnover events through the cofilin pathway [J].

Marshall, Thomas W. ;

Aloor, Heather L. ;

Bear, James E. .

JOURNAL OF CELL SCIENCE, 2009, 122 (17) :3061-3069

← 1 2 →