Directed nerve outgrowth is enhanced by engineered glial substrates

被引:60
作者
Biran, R
Noble, MD
Tresco, PA
机构
[1] Univ Utah, Dept Bioengn, Keck Ctr Tissue Engn, Salt Lake City, UT 84112 USA
[2] Univ Rochester, Sch Med, Dept Biomed Genet, Rochester, NY 14642 USA
关键词
neurite; guidance; astrocyte; oriented; neurite outgrowth; engineer; axon; glia; extracellular matrix; biomaterial;
D O I
10.1016/S0014-4886(03)00253-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the present study, the influence of astrocyte alignment on the direction and length of regenerating neurites was examined in vitro. Astrocytes were experimentally manipulated by different approaches to create longitudinally aligned monolayers. When cultured on the aligned monolayers, dorsal root ganglion neurites grew parallel to the long axis of the aligned astrocytes and were significantly longer than controls. Engineered monolayers expressed linear arrays of fibronectin, laminin, neural cell adhesion molecule, and chondroitin sulfate proteoglycan that were organized parallel to one another, suggesting that a particular spatial arrangement of these molecules on the astrocyte surface may be necessary to direct nerve regeneration in vivo. In contrast, no bias in directional outgrowth was observed for neurites growing on unorganized monolayers. The results suggest that altering the organization of astrocytes and their scar-associated matrix at the lesion site may be used to influence the direction and the length of adjacent regenerating axons in the damaged brain and spinal cord. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:141 / 152
页数:12
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