Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects

被引：77

作者：

Xue, Jing ^{[1
]}

Liang, Gemei ^{[1
]}

Crickmore, Neil ^{[2
]}

Li, Haitao ^{[1
]}

He, Kanglai ^{[1
]}

Song, Fuping ^{[1
]}

Feng, Xue ^{[1
]}

Huang, Dafang ^{[3
]}

Zhang, Jie ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China

[2] Univ Sussex, Sch Life Sci, Brighton BN1 9RH, E Sussex, England

[3] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100094, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2008年 / 280卷 / 01期

关键词：

Bacillus thuringiensis; novel cry1Ah gene; insecticidal activity; Ostrinia furnacalis; Helicoverpa armigera; Chilo suppressalis;

D O I：

10.1111/j.1574-6968.2007.01053.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain BT8. The toxin expressed in HD73(-) exhibited high toxicity against lepidopteran larvae of Ostrinia furnacalis, Helicoverpa armigera, Chilo suppressalis, and Plutella xylostella. The 50% lethal concentrations (LC(50)s) were 0.05, 1.48, 0.98 mu g g(-1) and 1.52 mu g mL(-1), respectively. The LC(50)s of Cry1Ah were significantly lower than that of Cry1Ac for H. armigera, C. suppressalis, and O. furnacalis, and lower than that of Cry1Ab and Cry1Ie for Ostrinia furnacalis. The high toxicity against a range of pest species makes this novel toxin a potential candidate for insect biocontrol.

引用

页码：95 / 101

页数：7

共 33 条

[1] Resistance to the Cry1Ac δ-endotoxin of Bacillus thuringiensis in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) [J].