Spectroscopic characterization of thiazole orange-3 DNA interaction

被引：22

作者：

Ghasemi, J. ^{[1
]}

Ahmadi, Sh. ^{[1
]}

Ahmad, A. I. ^{[2
]}

Ghobadi, S. ^{[3
]}

机构：

[1] Razi Univ, Dept Chem, Kermanshah, Iran

[2] Chalmers Univ Technol, Dept Chem & Biol Engn, S-41296 Gothenburg, Sweden

[3] Razi Univ, Dept Biol, Kermanshah, Iran

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2008年 / 149卷 / 01期

关键词：

ctDNA; TO-3; fluorescence; absorption spectra; MCR-ALS;

D O I：

10.1007/s12010-007-8124-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of a new derivative of thiazole orange (TO-3) with calf thymus DNA (ctDNA) has been investigated by fluorescence and absorption spectroscopy. When TO-3 binds to ctDNA, absorption bands exhibit significant hypochromicity at low base pair/dye ratio (BP/D ratio), and high BP/D show hyperchromicity with red shift. The spectral changes are attributed to the different species formed between TO-3 and ctDNA in the titration course of the dye molecule with DNA. Multivariate curve resolutions alternating least squares (MCR-ALS) is applied to the absorption measurements recorded to recover the concentration profiles and the pure spectra of the DNA/TO-3 complexes involved in the process. The binding constant and size of the binding site have been determined spectrophotometrically using the McGhee von Hippel equation. MCR-ALS has been used to reveal the precise concentration profiles of all detectable species formed between ctDNA and TO-3 and their pure spectral profiles.

引用

页码：9 / 22

页数：14

共 69 条

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