Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization

被引:598
作者
Licausi, Francesco [1 ,2 ]
Kosmacz, Monika [1 ]
Weits, Daan A. [1 ]
Giuntoli, Beatrice [2 ]
Giorgi, Federico M. [1 ]
Voesenek, Laurentius A. C. J. [3 ,4 ]
Perata, Pierdomenico [2 ]
van Dongen, Joost T. [1 ]
机构
[1] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany
[2] Scuola Super Sant Anna, Inst Life Sci, PlantLab, I-56127 Pisa, Italy
[3] Univ Utrecht, Inst Environm Biol, NL-3584 CH Utrecht, Netherlands
[4] Ctr Biosyst Genom, NL-6708 PB Wageningen, Netherlands
关键词
ETHYLENE RESPONSE FACTORS; EVOLUTIONARY CONSERVATION; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; BINDING PROTEIN; HYPOXIA; STRESS; LOCALIZATION; SYSTEM; CELLS;
D O I
10.1038/nature10536
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The majority of eukaryotic organisms rely on molecular oxygen for respiratory energy production(1). When the supply of oxygen is compromised, a variety of acclimation responses are activated to reduce the detrimental effects of energy depletion(2-4). Various oxygen-sensing mechanisms have been described that are thought to trigger these responses(5-9), but they each seem to be kingdom specific and no sensing mechanism has been identified in plants until now. Here we show that one branch of the ubiquitin-dependent N-end rule pathway for protein degradation, which is active in both mammals and plants(10,11), functions as an oxygen-sensing mechanism in Arabidopsis thaliana. We identified a conserved amino-terminal amino acid sequence of the ethylene response factor (ERF)-transcription factor RAP2.12 to be dedicated to an oxygen-dependent sequence of post-translational modifications, which ultimately lead to degradation of RAP2.12 under aerobic conditions. When the oxygen concentration is low-as during flooding-RAP2.12 is released from the plasma membrane and accumulates in the nucleus to activate gene expression for hypoxia acclimation. Our discovery of an oxygen-sensing mechanism opens up new possibilities for improving flooding tolerance in crops.
引用
收藏
页码:419 / U177
页数:5
相关论文
共 35 条
[1]   Flooding stress: Acclimations and genetic diversity [J].
Bailey-Serres, J. ;
Voesenek, L. A. C. J. .
ANNUAL REVIEW OF PLANT BIOLOGY, 2008, 59 :313-339
[2]   N-terminal processing:: the methionine aminopeptidase and Nα-acetyl transferase families [J].
Bradshaw, RA ;
Brickey, WW ;
Walker, KW .
TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (07) :263-267
[3]   PRT6/At5g02310 encodes an Arabidopsis ubiquitin ligase of the N-end rule pathway with arginine specificity and is not the CER3 locus [J].
Garzon, Marcus ;
Eifler, Karolin ;
Faust, Andrea ;
Scheel, Hartmut ;
Hofmann, Kay ;
Koncz, Csaba ;
Yephremov, Alexander ;
Bachmair, Andreas .
FEBS LETTERS, 2007, 581 (17) :3189-3196
[4]   New GATEWAY vectors for High Throughput Analyses of Protein-Protein Interactions by Bimolecular Fluorescence Complementation [J].
Gehl, Christian ;
Waadt, Rainer ;
Kudla, Joerg ;
Mendel, Ralf-R. ;
Haensch, Robert .
MOLECULAR PLANT, 2009, 2 (05) :1051-1058
[5]   The plant N-end rule pathway: structure and functions [J].
Graciet, Emmanuelle ;
Wellmer, Frank .
TRENDS IN PLANT SCIENCE, 2010, 15 (08) :447-453
[6]   Structure and evolutionary conservation of the plant N-end rule pathway [J].
Graciet, Emmanuelle ;
Mesiti, Francesca ;
Wellmer, Frank .
PLANT JOURNAL, 2010, 61 (05) :741-751
[7]   Bacterial sensors of oxygen [J].
Green, Jeffrey ;
Crack, Jason C. ;
Thomson, Andrew J. ;
LeBrun, Nick E. .
CURRENT OPINION IN MICROBIOLOGY, 2009, 12 (02) :145-151
[8]   The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water [J].
Hattori, Yoko ;
Nagai, Keisuke ;
Furukawa, Shizuka ;
Song, Xian-Jun ;
Kawano, Ritsuko ;
Sakakibara, Hitoshi ;
Wu, Jianzhong ;
Matsumoto, Takashi ;
Yoshimura, Atsushi ;
Kitano, Hidemi ;
Matsuoka, Makoto ;
Mori, Hitoshi ;
Ashikari, Motoyuki .
NATURE, 2009, 460 (7258) :1026-U116
[9]   Arabidopsis RAP2.2: An Ethylene Response Transcription Factor That Is Important for Hypoxia Survival [J].
Hinz, Manuela ;
Wilson, Iain W. ;
Yang, Jun ;
Buerstenbinder, Katharina ;
Llewellyn, Danny ;
Dennis, Elizabeth S. ;
Sauter, Margret ;
Dolferus, Rudy .
PLANT PHYSIOLOGY, 2010, 153 (02) :757-772
[10]   Myoglobin-like aerotaxis transducers in Archaea and Bacteria [J].
Hou, SB ;
Larsen, RW ;
Boudko, D ;
Riley, CW ;
Karatan, E ;
Zimmer, M ;
Ordal, GW ;
Alam, M .
NATURE, 2000, 403 (6769) :540-544