Improving membrane voltage measurements using FRET with new fluorescent proteins

被引：225

作者：

Tsutsui, Hidekazu ^{[1
]}

Karasawa, Satoshi ^{[1
,2
]}

Okamura, Yasushi ^{[3
]}

Miyawaki, Atsushi ^{[1
]}

机构：

[1] RIKEN, Brain Sci Inst, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan

[2] Amalgaam Co Ltd, Itabashi Ku, Tokyo 1730004, Japan

[3] Okazaki Inst Integrat Biosci, Sect Dev Neurophysiol, Okazaki, Aichi 4448585, Japan

来源：

NATURE METHODS | 2008年 / 5卷 / 08期

关键词：

D O I：

10.1038/nmeth.1235

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays similar to 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (similar to 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.

引用

页码：683 / 685

页数：3

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