Maintenance of replicative intermediates in ganciclovir-treated human cytomegalovirus-infected retinal glia

被引：9

作者：

Burd, EM

Pulido, JS

Puro, DG

OBrien, WJ

机构：

[1] MED COLL WISCONSIN,INST EYE,DEPT OPHTHALMOL,MILWAUKEE,WI 53226

[2] HENRY FORD HOSP,DEPT PATHOL,DETROIT,MI 48202

[3] UNIV MICHIGAN,DEPT OPHTHALMOL,ANN ARBOR,MI

[4] UNIV MICHIGAN,DEPT PHYSIOL,ANN ARBOR,MI

来源：

ARCHIVES OF OPHTHALMOLOGY | 1996年 / 114卷 / 07期

关键词：

D O I：

10.1001/archopht.1996.01100140070011

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Objectives: To characterize the molecular structure of the human cytomegalovirus (HCMV) DNA maintained in cultures of human retinal glia following ganciclovir treatment and to determine the biological activity of the DNA. Methods: Cultures of human retinal glia were established, infected with HCMV, treated with ganciclovir, and embedded in agarose, and the viral DNA was analyzed by field inversion gel electrophoresis. Results: The HCMV DNA was found to persist in cultures of infected, ganciclovir-treated retinal glial cells in the form of replicative intermediates. After removal of ganciclovir, processed forms of DNA in the 500- to 1000-kilobase range were found as well as 230-kb unit length genome. Infectious virus was recovered after termination of ganciclovir treatment. Conclusion: The data are consistent with the concept that ganciclovir's virostatic nature permits maintenance of HCMV DNA in retinal glia in a biologically active form that is capable of replication after removal of the drug.

引用

页码：856 / 861

页数：6

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