IL-1β stimulates argininosuccinate synthetase gene expression through NF-κB in Caco-2 cells

被引：16

作者：

Brasse-Lagnel, C ^{[1
]}

Lavoinne, A ^{[1
]}

Fairand, A ^{[1
]}

Vavasseur, K ^{[1
]}

Husson, A ^{[1
]}

机构：

[1] Univ Rouen, ADEN EA 3234, Fac Med Pharm Rouen, Grp Appareil Digest Environm & Nutr,Inst Fed Rech, F-76183 Rouen, France

来源：

BIOCHIMIE | 2005年 / 87卷 / 05期

关键词：

IL-1; beta; NF-kappa B; argininosuccinate synthetase; caco-2; cells;

D O I：

10.1016/j.biochi.2005.01.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Argininosuccinate synthetase (ASS) is limiting the arginine synthesis and can be stimulated by immunostimulants. We previously identified a putative NF-kappa B element in the human ASS gene promoter but its functionality was unknown (Husson et al., Eur. J. Biochem. 270 (2003) 1887). In the present study, using Caco-2 cells, a human enterocyte line, we demonstrate that IL-1 beta rapidly induces the expression of the ASS gene at a transcriptional level through NF-kappa B activation. Using gel shift assay and double-strand oligonucleotide sequence of the identified putative NF-kappa B binding site of the ASS promoter, we provide evidence that NF-kappa B may functionally interact with this element. (c) 2005 Elsevier SAS. All rights reserved.

引用

页码：403 / 409

页数：7

共 20 条

[1] SYNERGISTIC ACTIVATION OF A HUMAN PROMOTER INVIVO BY TRANSCRIPTION FACTOR SP1 [J].