Distribution of p63, cytokeratins 5/6 and cytokeratin 14 in 51 normal and 400 neoplastic human tissue samples using TARP-4 multi-tumor tissue microarray

被引:178
作者
Reis, JS
Simpson, PT
Martins, A
Preto, A
Gärtner, F
Schmitt, FC
机构
[1] Univ Porto, IPATIMUP Inst Mol Pathol & Immunol, P-4200 Oporto, Portugal
[2] Univ Porto, Fac Med, P-4100 Oporto, Portugal
[3] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst, Braga, Portugal
[4] Inst Canc Res, Breakthrough Toby Robins Breast Canc Res Ctr, London SW3 6JB, England
关键词
tissue microarray; immunohistochemisty; double immunostaining; squamous cell carcinoma; myoepithelial cell;
D O I
10.1007/s00428-003-0859-2
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
p63, cytokeratin (CK) 5/6 and CK 14 have been employed in diagnostic pathology as markers of basal, squamous and myoepithelial differentiation in several types of human neoplasms; however, there is scant data on the concurrent expression of these markers in large series of human neoplasms. We analyzed the distribution of these three immunohistochemical markers in 51 normal human tissue samples, 350 carcinomas, 25 malignant melanomas (MMs), and 25 glioblastomas using three serial sections of tissue array research program (TARP)-4 multi-tumor tissue microarray. Also, we performed double immunostainings to characterize the differential distribution of p63/CK 5/6 and p63/CK 14 in normal breast, salivary gland and skin. p63, CK 5/6 and CK 14 were expressed in basal cells of the prostate and respiratory epithelia and in breast and bronchial myoepithelial cells. p63 was also expressed in cytotrophoblast cells of human placenta and in scattered cells of lymph node germinal center. CK 5/6 and CK 14 also stained the cytoplasm of basal cells of esophageal stratified, squamous epithelium and transitional epithelial cells of the bladder. No mesenchymal, neural, endothelial, smooth muscle or adipose cells were stained by any of the markers. p63, CK 5/6, and CK 14 were respectively expressed in 92.6%, 75.0%, and 52.9% of the squamous cell carcinomas of the lung, 10.2%, 20.0%, and 7.4% of the ductal carcinomas of the breast, 12.9%, 34.4%, and 11.8% of the serous and 25.0%, 0%, and 0% of the endometrioid carcinomas of the ovary. Lung, prostate and colonic adenocarcinomas, as well as MMs and glioblastomas were only rarely decorated by one of the markers. Only matched samples of 16 squamous cell carcinomas and two ductal carcinomas of the breast co-expressed these three markers. In double immunostainings, p63-CK 5/6, as well as p63-CK 14 were co-expressed by basal/ myoepithelial cells of the salivary glands and basal cells of the epidermis. Our results demonstrate that p63, CK 5/ 6 and CK 14 may be used together in immunohistochemical panels to characterize squamous differentiation in poorly differentiated carcinomas or carcinomas of unknown origin.
引用
收藏
页码:122 / 132
页数:11
相关论文
共 48 条
  • [1] p63, a p53 homologue, is a selective nuclear marker of myoepithelial cells of the human breast
    Barbareschi, M
    Pecciarini, L
    Cangi, MG
    Macrì, E
    Rizzo, A
    Viale, G
    Doglioni, C
    [J]. AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 2001, 25 (08) : 1054 - 1060
  • [2] Common adult stem cells in the human breast give rise to glandular and myoepithelial cell lineages:: A new cell biological concept
    Böcker, W
    Moll, R
    Poremba, C
    Holland, R
    van Diest, PJ
    Dervan, P
    Bürger, H
    Wai, D
    Diallo, RI
    Brandt, B
    Herbst, H
    Schmidt, A
    Lerch, MM
    Buchwallow, IB
    [J]. LABORATORY INVESTIGATION, 2002, 82 (06) : 737 - 745
  • [3] Tissue microarray (TMA) technology:: miniaturized pathology archives for high-throughput in situ studies
    Bubendorf, L
    Nocito, A
    Moch, H
    Sauter, G
    [J]. JOURNAL OF PATHOLOGY, 2001, 195 (01) : 72 - 79
  • [4] Validation of tissue microarray technology in breast carcinoma
    Camp, RL
    Charette, LA
    Rimm, DL
    [J]. LABORATORY INVESTIGATION, 2000, 80 (12) : 1943 - 1949
  • [5] Differential expression of p53 gene family members p63 and p73 in head and neck squamous tumorigenesis
    Choi, HR
    Batsakis, JG
    Zhan, F
    Sturgis, E
    Luna, MA
    El-Naggar, AK
    [J]. HUMAN PATHOLOGY, 2002, 33 (02) : 158 - 164
  • [6] Keratin expression in human tissues and neoplasms
    Chu, PG
    Weiss, LM
    [J]. HISTOPATHOLOGY, 2002, 40 (05) : 403 - 439
  • [7] Cytokeratin 14 expression in epithelial neoplasms: a survey of 435 cases with emphasis on its value in differentiating squamous cell carcinomas from other epithelial tumours
    Chu, PG
    Lyda, MH
    Weiss, LM
    [J]. HISTOPATHOLOGY, 2001, 39 (01) : 9 - 16
  • [8] Expression of cytokeratin 5/6 in epithelial neoplasms: An immunohistochemical study of 509 cases
    Chu, PGG
    Weiss, LM
    [J]. MODERN PATHOLOGY, 2002, 15 (01) : 6 - 10
  • [9] Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura
    Cury, PM
    Butcher, DN
    Fisher, C
    Corrin, B
    Nicholson, AG
    [J]. MODERN PATHOLOGY, 2000, 13 (02) : 107 - 112
  • [10] p63 and p73 transactivate differentiation gene promoters in human keratinocytes
    De Laurenzi, V
    Rossi, A
    Terrinoni, A
    Barcaroli, D
    Levrero, M
    Costanzo, A
    Knight, RA
    Guerrieri, P
    Melino, G
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 273 (01) : 342 - 346