Fibrinolysis in human peritoneum during operation

被引:142
作者
Holmdahl, L [1 ]
Eriksson, E [1 ]
AlJabreen, M [1 ]
Risberg, B [1 ]
机构
[1] LUND UNIV,MALMO GEN HOSP,DEPT SURG,S-21401 MALMO,SWEDEN
关键词
D O I
10.1016/S0039-6060(96)80196-6
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. A reduced local fibrinolysis seems to be an important mechanism in the formation of adhesions. Peritonitis may cause adhesions, and the aim of the present study was to determine peritoneal fibrinolytic capacity in inflamed and normal peritoneum. Methods. Biopsy specimens from normal and inflamed human peritoneum were taken at the beginning and end of operation. After extraction plasminogen activator activity (PAA) was determined by using a chromogenic substrate assay in the presence or absence of inhibitory antibodies against tissue-type plasminogen activator (t-PA), urokinase of plasminogen activator inhibitor-1. Results. t-PA exerted 95% of the PAA. PAA was significantly reduced (p < 0.01) during peritonitis (3.0 IU/mu g protein; range 0.3 to 4.2) compared with normal peritoneum (7.1 IU/mu g protein; range, 0.6 to 18.1). A significant reduction (p < 0.05) in PAA occurred during operation both in normal peritoneum (3.8 IU/mu g protein; range, 0.8 to 8.6) and in peritonitis 0.6 IU/mu g protein; range, 0.16 to 2.1). Values are given as medians. Conclusions. The main PAA in human peritoneum was t-PA. The activity was decreasing during operation and reduced in peritonitis. This reduction in PAA might be a local response to inflammation.
引用
收藏
页码:701 / 705
页数:5
相关论文
共 27 条
[1]  
BAXTER GM, 1991, AM J VET RES, V52, P1121
[2]   HEMOSTATIC AND FIBRINOLYTIC PROPERTIES OF PERITONEAL-FLUID IN THE MENSTRUAL-CYCLE [J].
BOUCKAERT, PXJM ;
VANWERSCH, JWJ ;
SCHELLEKENS, LA ;
EVERS, JLH ;
ROLLAND, R .
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 1984, 91 (03) :256-259
[3]   UNIFYING PATHOGENETIC MECHANISM IN ETIOLOGY OF INTRAPERITONEAL ADHESIONS [J].
BUCKMAN, RF ;
WOODS, M ;
SARGENT, L ;
GERVIN, AS .
JOURNAL OF SURGICAL RESEARCH, 1976, 20 (01) :1-5
[4]  
DOODY KJ, 1989, FERTIL STERIL, V51, P509
[5]   PREVENTION OF POSTOPERATIVE ADHESIONS BY TISSUE-TYPE PLASMINOGEN-ACTIVATOR (T-PA) IN THE RABBIT [J].
DORR, PJ ;
VEMER, HM ;
BROMMER, EJP ;
WILLEMSEN, WNP ;
VELDHUIZEN, RW ;
ROLLAND, R .
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1990, 37 (03) :287-291
[6]   INTERLEUKIN-1 AND LIPOPOLYSACCHARIDE INDUCE AN INHIBITOR OF TISSUE-TYPE PLASMINOGEN-ACTIVATOR INVIVO AND IN CULTURED ENDOTHELIAL-CELLS [J].
EMEIS, JJ ;
KOOISTRA, T .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 163 (05) :1260-1266
[7]  
ERIKSSON E, 1989, THESIS U GOTEBORG
[8]   SEROSAL HYPOFIBRINOLYSIS - CAUSE OF POSTOPERATIVE ADHESIONS [J].
GERVIN, AS ;
PUCKETT, CL ;
SILVER, D .
AMERICAN JOURNAL OF SURGERY, 1973, 125 (01) :80-88
[9]  
HAU T, 1979, SURG GYNECOL OBSTET, V148, P415
[10]  
HOLMDAHL L, 1994, WOUND REPAIR REGEN, V7, P171