Texture Analysis of TEM Micrographs of Alginate Gels for Cell Microencapsulation

被引：9

作者：

Brun, Francesco ^{[1
,2
]}

Accardo, Agostino ^{[1
]}

Marchini, Maurizio ^{[3
]}

Ortolani, Fulvia ^{[3
]}

Turco, Gianluca ^{[4
]}

Paoletti, Sergio ^{[4
]}

机构：

[1] Univ Trieste, Dept Elect Elect & Comp Engn, I-34127 Trieste, Italy

[2] Sincrotrone Trieste SCpA, Trieste, Italy

[3] Univ Udine, Dept Med & Morphol Res, I-33100 Udine, Italy

[4] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2011年 / 74卷 / 01期

关键词：

alginate gels; image analysis; transmission electron microscopy (TEM); texture analysis; fractal analysis; FRACTAL DIMENSION; MICROCAPSULES; FEATURES; BEADS;

D O I：

10.1002/jemt.20874

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100123 [人体微生态学]; 100210 [外科学];

摘要：

In this work, the morphological characteristics of a calcium alginate gel and a binary (gel) mixture composed of (calcium) alginate and lactose-modified chitosan (chitlac) are evaluated and compared to quantify the differences between the two three-dimensional (3D) structures. A set of textural descriptors based on histogram analysis as well as on gray level co-occurrence matrix and on fractal dimension is extracted from transmission electron microscopy micrographs to describe the morphological differences that the images present. The obtained results reveal significant quantitative morphological differences between the calcium alginate gel and the binary gel mixture that were already inferred from rheological experiments, so as to provide a structural basis for developing new encapsulation systems based on such mixed polymer gels. Microsc. Res. Tech. 74:58-66, 2011. (C) 2010 Wiley-Liss, Inc.

引用

页码：58 / 66

页数：9

共 37 条

[1]

[Anonymous], TEXTURAL PROPERTIES

[2]

[Anonymous], STRUCTURAL ASPECTS R

[3]

[Anonymous], DIGITAL IMAGE PROCES

[4]

Image texture analysis: methods and comparisons [J].

Bharati, MH ;

Liu, JJ ;

MacGregor, JF .

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 2004, 72 (01) :57-71

[5]

Brun F, 2009, IFMBE PROC, V25, P157, DOI 10.1007/978-3-642-03900-3_46

[6]

Chapman JA., 1984, ULTRASTRUCTURE CONNE, P1

[7]

CLARKE KC, 1986, COMPUT GEOSCI, V12, P713, DOI 10.1016/0098-3004(86)90047-6

[8]

De Santis A., 1997, Ann. Geophys, V15, P811, DOI [10.4401/ag-3882, DOI 10.4401/AG-3882]

[9]

Synergistic effects in semidilute mixed solutions of alginate and lactose-modified chitosan (chitlac) [J].

Donati, Ivan ;

Haug, Ingvild J. ;

Scarpa, Tommaso ;

Borgogna, Massimiliano ;

Draget, Kurt I. ;

Skjak-Braek, Gudmund ;

Paoletti, Sergio .

BIOMACROMOLECULES, 2007, 8 (03) :957-962

[10]

Donati I, 2009, MICROBIOL MONOGR, V13, P1, DOI 10.1007/978-3-540-92679-5_1

← 1 2 3 4 →