Epithelial Cell Extrusion Zones Observed on Confocal Laser Endomicroscopy Correlates with Immunohistochemical Staining of Mucosal Biopsy Samples

被引：11

作者：

Liu, Julia J. ^{[1
,2
,7
]}

Kay, Theresa M. ^{[3
]}

Davis, Elisabeth M. ^{[1
,2
]}

Lou, Yuefei ^{[4
]}

Kao, Dina ^{[4
]}

Claggett, Brian ^{[5
]}

Fedorak, Richard N. ^{[4
]}

Irvin, Randall T. ^{[6
]}

机构：

[1] Univ Arkansas Med Sci, Div Gastroenterol, Little Rock, AR 72205 USA

[2] Cent Arkansas Vet Healthcare Syst, Little Rock, AR USA

[3] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[4] Univ Alberta, Dept Med, Div Gastroenterol, Edmonton, AB, Canada

[5] Brigham & Womens Hosp, Dept Med, 75 Francis St, Boston, MA 02115 USA

[6] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB, Canada

[7] Univ Arkansas Med Sci, Div Gastroenterol & Hepatol, Shorey S8-62, Little Rock, AR 72205 USA

来源：

DIGESTIVE DISEASES AND SCIENCES | 2016年 / 61卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Confocal laser endomicroscopy; Small intestine; Epithelial extrusion zones; epithelial gaps; Inflammatory bowel disease; Irritable bowel syndrome; BARRIER FUNCTION; GAPS; INTESTINE; RELAPSE; DISEASE;

D O I：

10.1007/s10620-016-4154-x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

The density of epithelial cell extrusion zones in the intestinal lining, also known as gap density (number of gaps/1000 epithelial cells counted), can be quantitated using probe-based confocal laser endomicroscopy (pCLE). Gap density has been reported to be higher than normal in both inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) patients. Epithelial cells destined for extrusion from the intestinal surface would stain positive for either activated caspase-1 or caspase-3 on mucosal biopsy samples. The aim of this study was to determine whether epithelial gap density on pCLE correlates with quantitative analysis of activated caspase staining of mucosal biopsy samples from patients. We obtained pCLE images and biopsy samples of the terminal ileum during colonoscopies of healthy controls and patients with either IBD or IBS. The pCLE images and biopsy samples were blindly analyzed for gap density and for cells staining positive for activated caspases, respectively. The degree of correlation was determined using nonparametric statistical tests. The median results were 10 gaps/1000 cells counted for controls versus 33 gaps/1000 cells counted for chronic intestinal disorder patients (p = 0.02). Activated caspase staining showed 13 positive cells/1000 epithelial cells counted versus 26 positive cells/1000 epithelial cells counted, respectively (p = 0.02), thus showing a strong correlation with a Spearman's coefficient rho of 0.61 (strong correlation for rho = 0.4-0.75, p = 0.01). Intestinal epithelial gap density via pCLE correlated strongly with quantitative analysis of immunohistochemical staining of mucosal biopsy samples.

引用

页码：1895 / 1902

页数：8

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