Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase

被引：11

作者：

Morita, Hiroyuki ^{[2
]}

Kondo, Shin ^{[1
]}

Kato, Ryohei ^{[1
]}

Wanibuchi, Kiyofumi ^{[3
]}

Noguchi, Hiroshi ^{[3
]}

Sugio, Shigetoshi ^{[1
]}

Abe, Ikuro ^{[3
,4
]}

Kohno, Toshiyuki ^{[2
]}

机构：

[1] Mitsubishi Chem Corp, Innovat Ctr Yokohama, Aoba Ku, Yokohama, Kanagawa 2278502, Japan

[2] MITILS, Tokyo 1948511, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan

[4] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S1744309107047069

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 angstrom, alpha = beta = gamma = 90.0 degrees. Diffraction data were collected to 2.6 angstrom resolution using synchrotron radiation at BL24XU of SPring-8.

引用

页码：947 / 949

页数：3

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