Protective role of zinc-metallothionein on DNA damage in vitro by ferric nitrilotriacetate (Fe-NTA) and ferric salts

被引：50

作者：

Cai, L ^{[1
]}

Tsiapalis, G ^{[1
]}

Cherian, MG ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Pathol, London, ON N6A 5C1, Canada

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1998年 / 115卷 / 02期

基金：

英国医学研究理事会;

关键词：

metallothionein; oxidative DNA damage; iron complexes; transitional metals;

D O I：

10.1016/S0009-2797(98)00069-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative DNA damage can be caused by radicals generated by transitional metals like iron in Fenton reaction. Metallothionein (MT) may play an important role in preventing oxidative DNA damage. Therefore, after comparing the effects of ferric salts (Fe), and complexes of ferric salts with nitrilotriacetic acid (Fe-NTA) on DNA damage, the protective effects of zinc-MT (Zn-MT) on DNA damage of Fe salts or Fe-NTA were investigated in vitro. DNA damage was measured by loss of fluorescence of DNA binding to ethidium bromide, and also by increased DNA mobility in agarose gel electrophoresis. Both Fe salts and Fe-NTA could induce calf thymus DNA damage in presence of hydrogen peroxide and ascorbate. However, the degree of DNA damage was lower with Fe salts than that with Fe-NTA complex. Addition of 50 mu M Zn-MT could only protect DNA from Fe-NTA, but not from Fe salt induced damage. The protective effect of MT was about five times better than that of glutathione (GSH). These results suggest a potential role for MT in protection from Fe-NTA-induced DNA damage. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：141 / 151

页数：11

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