Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF

The yeast Saccharomyces cerevisiae contains two related chromatin-remodeling complexes, RSC and SWI/SNF, which are shown to share the actin-related proteins Arp7 and Arp9. Depending on the genetic background tested, arp7 Delta and arp9 Delta mutants are either inviable or show greatly impaired growth and Swi(-)/Snf(-) mutant phenotypes. Unlike swi(-)/snf(-) mutants, viable arp7 Delta or arp9 Delta mutants have an Spt(-) phenotype, suggesting that RSC affects transcription. Temperature-sensitive mutations in ARP7 and ARP9 were isolated, and the amino acid changes support the structural relationship of Arp7 and Arp9 to actin. However, site-directed mutations predicted to impair ATP binding or hydrolysis did not detectably affect Arp7 or Arp9 function. Our results suggest that actin-related proteins perform important roles in chromatin-remodeling complexes by virtue of structural rather than enzymatic similarities to actin.