Determination of active component in silymarin by RP-LC and LC/MS

被引:99
作者
Ding, TM [1 ]
Tian, SJ
Zhang, ZX
Gu, DZ
Chen, YF
Shi, YH
Sun, ZP
机构
[1] Hubei Inst Drug Control, Wuhan 430064, Peoples R China
[2] Natl Inst Control Pharmaceut & Biol Prod, Div Instrumental Anal, Beijing 100050, Peoples R China
[3] China Pharmaceut Univ, Nanjing 210009, Peoples R China
关键词
diastereoisomers; silymarin; LC/MS; RP-LC;
D O I
10.1016/S0731-7085(01)00364-8
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Silybin, isosilybin, silydianin and silycristin in silymarin were separated and quantitatively determined by RP-HPLC. Two diastereoisomers of silybin and isosilybin were respectively separated by RP-HPLC and confirmed by LC/MS. Chromatographic condition consisted of column: Shim-pack VP-ODS (150 x 4.6 mm i.d. 5 mum) and Pre-column (10 x 4.6 mm i.d. 5 mum); mobile phase: methanol and solvent mixture (water: dioxane = 9:1) by gradient; flow rate: 1.5 ml/min; column temp.: 40 degreesC; detector wavelength: 288 nm; The recovery of 99.66% for silycristin, 99.48% for silydianin. 100.0% for silybin and 98.72% for isosilybin was respectively obtained. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:155 / 161
页数:7
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