Transcriptional response of a human colon adenocarcinoma cell line to sodium butyrate

被引：69

作者：

Iacomino, G

Tecce, MF

Grimaldi, C

Tosto, M

Russo, GL

机构：

[1] CNR, Ist Sci Alimentazione, I-83100 Avellino, Italy

[2] Univ Bari, Dipartimento Farmaco Biol, I-70010 Bari, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 285卷 / 05期

关键词：

butyrate; gene array; colon cancer; HT29 cell line;

D O I：

10.1006/bbrc.2001.5323

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taking advantage of the DNA array screening technology, we analysed the effect of sodium butyrate on mRNA transcription in human HT29 colon adenocarcinoma cells. Out of 588 mRNA species analysed, only 119 resulted expressed. Among these, 60 exhibited a variable degree of modulation after butyrate treatment. Genes linked to the cell growth, apoptosis and oxidative metabolism appeared the most significantly affected. Furthermore, many of the differentially expressed genes are transcription factors and this may account for the variability of the biological effects of butyrate. The pattern of butyrate-affected genes may represent a reference in further analyses of gene expression of intestinal cells and tissues. (C) 2001 Academic Press.

引用

页码：1280 / 1289

页数：10

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