Bi-functional gfp-and gusA-containing mini-Tn5 transposon derivatives for combined gene expression and bacterial localization studies

被引:96
作者
Xi, CW [1 ]
Lambrecht, M [1 ]
Vanderleyden, J [1 ]
Michiels, J [1 ]
机构
[1] Katholieke Univ Leuven, FA Janssens Lab Genet, B-3001 Heverlee, Belgium
关键词
GFP; beta-glucuronidase; mini-Tn5; transposons; Rhizobium; Azospirillum; Pseudomonas;
D O I
10.1016/S0167-7012(98)00103-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The gfp gene, encoding the green fluorescent protein, was combined with the gusA gene, coding for the beta-glucuronidase enzyme, in mini-Tn5 transposon derivatives for use in Gram-negative bacteria. These mini-Tn5 elements allow simultaneously monitoring of gene expression and localization of the marked bacteria. Introduction of the resultant mini-Tn5 transposons into Rhizobium etli, Azospirillum brasilense and Pseudomonas stutzeri allowed us to visualise the interaction of these bacteria with their host plant. The dual-marker mini-Tn5 transposons constitute a powerful new tool for studying gene expression and ecology of bacteria in the environment and during the interaction with plants. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:85 / 92
页数:8
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