Analysis of the methane production in thermophilic anaerobic reactors: use of autofluorescence microscopy

被引:18
作者
Solera, R [1 ]
Romero, LI [1 ]
Sales, D [1 ]
机构
[1] Univ Cadiz, Fac Marine & Environm Sci, Dept Chem Engn Food & Environm Technol, Cadiz 11510, Spain
关键词
activity; anaerobic process; biogas; methanogens; waste water treatment;
D O I
10.1023/A:1012762015659
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Methanogenic activity in thermophilic, anaerobic reactors was determined by comparing the amount of methane generated in single- and two-stage systems with the size of the methanogenic population, as determined by microscopy. The methanogenic activities were 2.71 x 10(-9) ml methane cell(-1) d(-1) and 1.10 x 10(-9) ml methane cell(-1) d(-1) for 10 and 4 days of the hydraulic retention time (HRT), in the single-stage system. In the two-stage system, 7.49 x 10(-9) ml methane cell(-1) d(-1) in the acidogenic reactor and 1.56 x 10(-9) ml methane cell(-1) d(-1) in the methanogenic reactor for 4 days of the HRT. A high correlation was evident between the methane production and methanogenic population [0.1354 ln(x) - 2.1375](R-2 0.8619).
引用
收藏
页码:1889 / 1892
页数:4
相关论文
共 11 条
[1]  
CLESCERLI LS, 1990, STANDARD METHODS EXA
[2]   IMPROVED IDENTIFICATION OF METHANOGENIC BACTERIA BY FLUORESCENCE MICROSCOPY [J].
DODDEMA, HJ ;
VOGELS, GD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 36 (05) :752-754
[3]   COLONIZATION OF POLYURETHANE RETICULATED FOAM BIOMASS SUPPORT PARTICLE BY METHANOGEN SPECIES [J].
FYNN, GH ;
WHITMORE, TN .
BIOTECHNOLOGY LETTERS, 1982, 4 (09) :577-582
[4]  
Jain M. K., 1991, ANAEROBIC MICROBIOLO, P223
[5]  
Jawed M, 1999, WATER SA, V25, P345
[6]   BIOFILM CHARACTERIZATION AND ACTIVITY ANALYSIS IN WATER AND WASTE-WATER TREATMENT [J].
LAZAROVA, V ;
MANEM, J .
WATER RESEARCH, 1995, 29 (10) :2227-2245
[7]   EFFECT OF THE FEED FREQUENCY ON THE PERFORMANCE OF ANAEROBIC FILTERS [J].
NEBOT, E ;
ROMERO, LI ;
QUIROGA, JM ;
SALES, D .
ANAEROBE, 1995, 1 (02) :113-120
[8]  
Perez M, 1997, CHEM BIOCHEM ENG Q, V11, P133
[9]  
SHANG Y, 1998, P WATER ENV FED ANN, V71, P431
[10]   Determination of the microbial population in thermophilic anaerobic reactor: Comparative analysis by different counting methods [J].
Solera, R ;
Romero, LI ;
Sales, D .
ANAEROBE, 2001, 7 (02) :79-86