A preformed compact ribosome-binding domain in the cricket paralysis-like virus IRES RNAs

被引:62
作者
Costantino, D [1 ]
Kieft, JS [1 ]
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Biochem & Mol Genet, Aurora, CO 80045 USA
关键词
internal ribosome entry site (IRES); translation; CrPV-like; RNA folding; RNA structure;
D O I
10.1261/rna.7184705
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The internal ribosome site RNA of the cricket paralysis-like viruses (CrPV-Iike) binds directly to the ribosome, assembling the translation machinery without initiation factors. This mechanism does not require initiator tRNA, and translation starts from a non-AUG codon. A wealth of biochemical data has yielded a working model for this process, but the three-dimensional structure and biophysical characteristics of the unbound CrPV-Iike IRES RNAs are largely unexplored. Here, we demonstrate that the CrPV-Iike IRESes prefold into a two-part structure in the presence of magnesium ions. The largest part is a prefolded compact RNA domain that shares folding and structural characteristics with other compactly folded RNAs such as group I intron RNAs and RNase P RNA. Chemical probing reveals that the CrPV-Iike IRES' compact domain contains RNA helices that are packed tightly enough to exclude solvent, and analytical ultracentrifugation indicates a large change in the shape of the IRES upon folding. Formation of this compact domain is necessary for binding of the 40S subunit, and the structural organization of the unbound IRES RNA is consistent with the hypothesis that the IRES is functionally and structurally preorganized before ribosome binding.
引用
收藏
页码:332 / 343
页数:12
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