Evaluation of the structure/cross-reactivity relationship of polycyclic aromatic compounds using an enzyme-linked immunosorbent assay kit

被引:14
作者
Nording, M [1 ]
Haglund, P
机构
[1] Umea Univ, Dept Chem, SE-90187 Umea, Sweden
[2] Swedish Def Res Agcy, SE-90182 Umea, Sweden
关键词
cross-reactivity; PAH; immunoassay; ELISA; SARs;
D O I
10.1016/S0003-2670(03)00466-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A commercially available enzyme-linked immunosorbent assay (ELISA) kit, the PAH RIS(R) soil test, was evaluated with regard to cross-reactivity. Phenanthrene in methanol was used as reference substance. Anthracene, naphthalene and fluorene were chosen as representatives of the 16 US-EPA priority-pollutant polycyclic aromatic hydrocarbons (PAHs). In addition, a number of polycyclic aromatic compounds (PACs), including methyl-, phenyl-, and carbonyl-PAHs, as well as NSO-heterocyclic PACs, found at former industrial sites, were chosen for elucidation of structure/cross-reactivity relationships. The study emphasizes the importance of a priori knowledge of sample composition for accurate interpretation of test results. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:43 / 50
页数:8
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