Generation, identification, and evaluation of expressed sequence tags from different developmental stages of the Asian blood fluke Schistosoma japonicum

被引：34

作者：

Fan, JJ ^{[1
]}

Minchella, DJ

Day, SR

McManus, DP

Tiu, WU

Brindley, PJ

机构：

[1] Queensland Inst Med Res, Mol Parasitol Unit, Brisbane, Qld 4006, Australia

[2] Queensland Inst Med Res, Australian Ctr Int & Trop Hlth & Nutr, Brisbane, Qld 4006, Australia

[3] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[4] Univ Philippines, Coll Publ Hlth, Manila, Philippines

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 252卷 / 02期

关键词：

schistosome; Schistosoma japonicum; expressed sequence tags; genome;

D O I：

10.1006/bbrc.1998.9491

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here me report 658 expressed sequence tags (ESTs) generated from the 5'-termini of clones randomly selected from directional cDNA libraries constructed from mRNAs from three developmental stages of Schistosoma japonicum. Putative identifications were assigned to 46.2% of the ESTs; 6.4% were previously known from S. japonicum, 5.6% were previously known from S. mansoni, 34.2% were known from other organisms, and the remaining 53.8% may represent S. japonicum-specific genes. These 658 ESTs appeared to be derived from 457 unique genes, which together represent 2 to 3% of the 15,000 to 20,000 genes predicted to occur in the schistosome genome. (C) 1998 Academic Press.

引用

页码：348 / 356

页数：9

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