Distinct phospholipase C-regulated signalling pathways in Swiss 3T3 fibroblasts induce the rapid generation of the same polyunsaturated diacylglycerols

被引:16
作者
Pettitt, TR [1 ]
Wakelam, MJO [1 ]
机构
[1] Univ Birmingham, Sch Med, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England
基金
英国惠康基金;
关键词
cell signalling; diacylglycerol; phospholipase C; protein kinase C; Swiss; 3T3;
D O I
10.1016/S0014-5793(98)00471-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prostaglandin F-2 alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/120:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts,This was followed by a second sustained phase characterised by saturated, monounsaturated and diunsaturated DAG species derived, at least partially, from a phospholipase D/phosphatidate phosphohydrolase-linked pathway. This could be directly activated by phorbol ester, Assay of rat brain protein kinase C (PKC) in lipid vesicles showed that first phase, polyunsaturated-enriched BAG isolated from Swiss 3T3 cells was a more potent activator of kinase activity compared to that achieved with DAG from control or 5 min stimulated cells. Thus activation of distinct members of the phospholipase C family leads to the rapid and almost identical generation of polyunsaturated DAG species which are capable of preferentially activating protein kinase C (PKC). (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:371 / 376
页数:6
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