Bufalin influences the repair of X-ray-induced DNA breaks in Chinese hamster cells

被引：31

作者：

Pastor, N ^{[1
]}

Cortés, F ^{[1
]}

机构：

[1] Univ Seville, Fac Biol, Dept Cell Biol, E-41012 Seville, Spain

来源：

DNA REPAIR | 2003年 / 2卷 / 12期

关键词：

DNA radiation damage repair; topoisomerase II; bufalin; comet assay;

D O I：

10.1016/j.dnarep.2003.08.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The bufadienolide bufalin, a component of the Chinese medicine chan'su, has been reported to selectively inhibit the growth of various lines of human cancer cells, due at least in part to its specific effect on topoisomerase (topo) II. We have treated Chinese hamster ovary (CHO) cells with doses of bufalin that result in a dramatic reduction in both the level and catalytic activity of topo II without any concomitant induction of DNA damage, as assessed by the comet assay. When cells were pre-treated with bufalin and then irradiated with X-rays, a follow-up study revealed that the kinetics of DNA repair was clearly affected, with a general delay in the restoration of DNA to the situation observed in non-irradiated controls. The possible involvement of topo II in radiation damage repair is discussed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1353 / 1360

页数：8

共 30 条

[1] Catalytic inhibitors of DNA topoisomerase II [J].