Androgen receptors in human preadipocytes and adipocytes:: regional specificities and regulation by sex steroids

被引:165
作者
Dieudonné, MN [1 ]
Pecquery, R [1 ]
Boumediene, A [1 ]
Leneveu, MC [1 ]
Giudicelli, Y [1 ]
机构
[1] Univ Rene Descartes, Ctr Hosp, Fac Med Paris Ouest, Serv Biochim,INSERM CJF 9402, F-78303 Poissy, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1998年 / 274卷 / 06期
关键词
adipogenesis; sex hormones; cultured preadipocytes;
D O I
10.1152/ajpcell.1998.274.6.C1645
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Various clinical and epidemiological evidence strongly suggests a major role for sex steroid hormones in the determination of anatomical specificities of fat distribution in human. To date, no studies have examined the possible presence of androgen receptors (AR) in human adipocytes and preadipocytes. We, have studied AR in preadipocytes from various anatomical locations (intra-abdominal and subcutaneous) in middle-aged men and women during the proliferation and differentiation processes (adipogenesis). Androgen binding sites quantified by [H-3]R-1881-specific binding in whole cell extracts were twofold higher in intraabdominal than in subcutaneous preadipocytes but identical for the same fat depots in men and women. Western blot analysis revealed 1) the presence of AR in the nuclear and cytosolic fractions of human preadipocytes, 2) a decrease of AR expression during adipogenesis, and 3) an upregulation of AR by androgens in vitro. RT-PCR experiments showed the presence of AR mRNA in human preadipocytes and adipocytes and also the regional specificity of AR distribution. However, AR mRNA expression was found to increase during adipogenesis. The same results were observed in rat preadipocytes. In conclusion, this study clearly demonstrates the presence of AR in human preadipocytes and adipocytes and suggests that androgens may contribute, through regulation of their own receptors, to the control of adipose tissue development.
引用
收藏
页码:C1645 / C1652
页数:8
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