Involvement of NF-AT in type I human T-cell leukemia virus tax-mediated Fas ligand promoter transactivation

Human T-cell leukemia virus type I (HTLV-I)-infected T-cells constitutively express surface Fas ligand (FasL), which may serve as a mechanism of viral pathogenesis. HTLV-I induces transcription of FasL gene through the viral transactivator Tax, although the underlying molecular mechanism remains unclear. In the present study, we have analyzed both the cis-activating element and transactivating factors involved in Tax activation of the Fast promoter. We show that the 486-base pair upstream region of the human Fast gene is sufficient for Tax-mediated transactivation in Jurkat T-cells. Interestingly, a palindromic DNA sequence (GGAAACTTCC) covering the consensus NF-ATp binding site (GGAAA), is required for Tax activation of Fast promoter. The involvement of NF-AT in this transactivation event is suggested by the finding that Tax fails to activate the Fast promoter in a Jurkat T-cell line defective in capacitative calcium entry, a signaling mechanism involved in NF-AT activation. Furthermore, activation of Fast promoter by Tax is largely attenuated in the nonlymphoid F9 embryonal and COS kidney cells deficient in NF-ATE, expression. DNA-protein interaction assays reveal that the NF-AT-like motif in the Fast promoter is bound by both NF-ATp and NF-AT4 in Jurkat T-cells expressing Tax. The binding of NF-ATp, although not NF-AT4, to this enhancer also occurs along with HTLV-I-mediated infection of human peripheral blood T-cells. Furthermore, exogenously transfected NF-ATp binds to the NF-AT-like enhancer and participates in Tax-mediated Fast promoter transactivation. These results suggest an important role for proteins of the NF-AT family in HTLV-1 Tax-mediated Fast gene transactivation.