Peroxiredoxin 2 overexpression protects cortical neuronal cultures from ischemic and oxidative injury but not glutamate excitotoxicity, whereas Cu/Zn superoxide dismutase 1 overexpression protects only against oxidative injury

被引:49
作者
Boulos, Sherif
Meloni, Bruno P.
Arthur, Peter G.
Bojarski, Christina
Knuckey, Neville W.
机构
[1] Univ Western Australia, QEII Med Ctr, Australian Neuromuscular Res Inst, Ctr Neuromuscular & Neurol Disorders, Nedlands, WA 6009, Australia
[2] Univ Western Australia, Sch Biomed & Chem Sci, Nedlands, WA 6009, Australia
[3] Sir Charles Gairdner Hosp, Dept Neurosurg, Nedlands, WA 6009, Australia
关键词
neuroprotection; cumene hydroperoxide; oxygen-glucose deprivation; in vitro; adenovirus;
D O I
10.1002/jnr.21429
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We previously reported that peroxiredoxin 2 (PRDX2) and Cu/Zn superoxide dismutase 1 (SOD1) proteins are up-regulated in rat primary neuronal cultures following erythropoietin (EPO) preconditioning. In the present study, we have demonstrated that adenovirally mediated overexpression of PRDX2 in cortical neuronal cultures can protect neurons from in vitro ischemia (oxygen-glucose deprivation) and an oxidative insult (cumene hydroperoxide) but not glutamate excitotoxicity. We have also demonstrated that adenovirally mediated overexpression of SOD1 in cortical neuronal cultures protected neurons only against the oxidative insult. Interestingly, we did not detect up-regulation of PRDX2 or SOD1 protein in the rat hippocampus following exposure to either 3 min or 8 min of global cerebral ischemia. Further characterization of PRDX2's neuroprotective mechanisms may aid in the development of a neuroprotective therapy. (c) 2007 Wiley-Liss, Inc.
引用
收藏
页码:3089 / 3097
页数:9
相关论文
共 66 条
[1]  
[Anonymous], FREE RADICALS BIOL M
[2]   The protective effect of hypoxic preconditioning on cortical neuronal cultures is associated with increases in the activity of several antioxidant enzymes [J].
Arthur, PG ;
Lim, SCC ;
Meloni, BP ;
Munns, SE ;
Chan, A ;
Knuckey, NW .
BRAIN RESEARCH, 2004, 1017 (1-2) :146-154
[3]   1-methyl-4-phenylpyridinium neurotoxicity is attenuated by adenoviral gene transfer of human Cu/Zn superoxide dismutase [J].
Barkats, M ;
Horellou, P ;
Colin, P ;
Millecamps, S ;
Faucon-Biguet, N ;
Mallet, J .
JOURNAL OF NEUROSCIENCE RESEARCH, 2006, 83 (02) :233-242
[4]   An adenovirus encoding CuZnSOD protects cultured striatal neurones against glutamate toxicity [J].
Barkats, M ;
Bemelmans, AP ;
Geoffroy, MC ;
Robert, JJ ;
Loquet, I ;
Horellou, P ;
Revah, F ;
Mallet, J .
NEUROREPORT, 1996, 7 (02) :497-501
[5]   Neuronal transfer of the human Cu/Zn superoxide dismutase gene increases the resistance of dopaminergic neurons to 6-hydroxydopamine [J].
Barkats, M ;
Millecamps, S ;
Bilang-Bleuel, A ;
Mallet, J .
JOURNAL OF NEUROCHEMISTRY, 2002, 82 (01) :101-109
[6]   Proteome analysis of human substantia nigra in Parkinson's disease [J].
Basso, M ;
Giraudo, S ;
Corpillo, D ;
Bergamasco, B ;
Lopiano, L ;
Fasano, M .
PROTEOMICS, 2004, 4 (12) :3943-3952
[7]   Copper/zinc superoxide dismutase overexpression promotes survival of cortical neurons exposed to neurotoxins in vitro [J].
Borg, J ;
London, J .
JOURNAL OF NEUROSCIENCE RESEARCH, 2002, 70 (02) :180-189
[8]   Evidence that intracellular cyclophilin A and cyclophilin A/CD147 receptor-mediated ERK1/2 signalling can protect neurons against in vitro oxidative and ischemic injury [J].
Boulos, Sherif ;
Meloni, Bruno P. ;
Arthur, Peter G. ;
Majda, Bernadette ;
Bojarski, Christina ;
Knuckey, Neville W. .
NEUROBIOLOGY OF DISEASE, 2007, 25 (01) :54-64
[9]   Assessment of CMV, RSV and SYN1 promoters and the woodchuck post-transcriptional regulatory element in adenovirus vectors for transgene expression in cortical neuronal cultures [J].
Boulos, Sherif ;
Meloni, Bruno P. ;
Arthur, Peter G. ;
Bojarski, Christina ;
Knuckey, Neville W. .
BRAIN RESEARCH, 2006, 1102 :27-38
[10]   Peroxynitrite reductase activity of bacterial peroxiredoxins [J].
Bryk, R ;
Griffin, P ;
Nathan, C .
NATURE, 2000, 407 (6801) :211-215