The sulfate transporter SST1 is crucial for symbiotic nitrogen fixation in Lotus japonicus root nodules

被引:170
作者
Krusell, L
Krause, K
Ott, T
Desbrosses, G
Krämer, U
Sato, S
Nakamura, Y
Tabata, S
James, EK
Sandal, N
Stougaard, J
Kawaguchi, M
Miyamoto, A
Suganuma, N
Udvardi, MK [1 ]
机构
[1] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany
[2] Kazusa DNA Res Inst, Chiba 2920812, Japan
[3] Univ Dundee, Sch Life Sci, Dundee DD1 5EH, Scotland
[4] Univ Aarhus, Dept Mol Biol, DK-8000 Aarhus, Denmark
[5] Univ Tokyo, Dept Biol Sci, Tokyo 1130033, Japan
[6] Aichi Univ Educ, Dept Life Sci, Aichi 4488542, Japan
关键词
D O I
10.1105/tpc.104.030106
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Symbiotic nitrogen fixation (SNF) by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. Little is known at the molecular level about plant transporters that mediate such exchanges. Several mutants of the model legume Lotus japonicus have been identified that develop nodules with metabolic defects that cannot fix nitrogen efficiently and exhibit retarded growth under symbiotic conditions. Map-based cloning of defective genes in two such mutants, sst1-1 and sst1-2 (for symbiotic sulfate transporter), revealed two alleles of the same gene. The gene is expressed in a nodule-specific manner and encodes a protein homologous with eukaryotic sulfate transporters. Full-length cDNA of the gene complemented a yeast mutant defective in sulfate transport. Hence, the gene was named Sst1. The sst1-1 and sst1-2 mutants exhibited normal growth and development under nonsymbiotic growth conditions, a result consistent with the nodule-specific expression of Sst1. Data from a previous proteomic study indicate that SST1 is located on the symbiosome membrane in Lotus nodules. Together, these results suggest that SST1 transports sulfate from the plant cell cytoplasm to the intracellular rhizobia, where the nutrient is essential for protein and cofactor synthesis, including nitrogenase biosynthesis. This work shows the importance of plant sulfate transport in SNF and the specialization of a eukaryotic transporter gene for this purpose.
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页码:1625 / 1636
页数:12
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