B and T lymphocyte attenuator exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T cells

被引:154
作者
Hurchla, MA
Sedy, JR
Gavrielli, M
Drake, CG
Murphy, TL
Murphy, KM
机构
[1] Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Ctr Immunol, St Louis, MO 63110 USA
[3] Howard Hughes Med Inst, St Louis, MO 63110 USA
[4] Johns Hopkins Med Inst, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA
关键词
D O I
10.4049/jimmunol.174.6.3377
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
B and T lymphocyte attenuator (BTLA) was initially identified as expressed on Th1 cells and B cells, but recently reported to be expressed by macrophages, dendritie cells, and NK cells as well. To address this discrepancy we generated a panel of BTLA-specific mAbs and characterized BTLA expression under various activation conditions. We report the existence of three distinct BTLA alleles among 23 murine strains, differing both in Ig domain structure and cellular distribution of expression on lymphoid subsets. The BALB/c and MRL/lpr alleles differ at one amino acid residue, but C57BL/6 has nine additional differences and alters the predicted cysteine bonding pattern. The BALB/c BTLA allele is also expressed by B cells, T cells, and dendritic cells, but not macrophages or NK cells. However, C57BL/6 BTLA is expressed on CD11b(+) macrophages and NK cells. Finally, in CD4(+) T cells, BTLA is expressed most highly following Ag-specific induction of anergy in vivo, and unlike programmed death-1 and CTLA-4, not expressed by CD25(+) regulatory T cells. These results clarify discrepancies regarding BTLA expression, suggest that structural and expression polymorphisms be considered when analyzing BTLA in various murine backgrounds, and indicate a possible role in anergic CD4(+) T cells.
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收藏
页码:3377 / 3385
页数:9
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