An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells

被引：67

作者：

Castelnuovo, Manuele ^{[1
,3
]}

Massone, Sara ^{[1
,3
]}

Tasso, Roberta ^{[1
,3
]}

Fiorino, Gloria ^{[4
]}

Gatti, Monica ^{[1
]}

Robello, Mauro ^{[2
]}

Gatta, Elena ^{[2
]}

Berger, Audrey ^{[5
]}

Strub, Katharina ^{[5
]}

Florio, Tullio ^{[1
]}

Dieci, Giorgio ^{[4
]}

Cancedda, Ranieri ^{[1
,3
]}

Pagano, Aldo ^{[1
,3
]}

机构：

[1] Univ Genoa, Dept Oncol Biol & Genet, I-16132 Genoa, Italy

[2] Univ Genoa, Dept Phys, I-16132 Genoa, Italy

[3] Natl Inst Canc Res Genoa, Genoa, Italy

[4] Univ Parma, Dept Biochem & Mol Biol, I-43100 Parma, Italy

[5] Univ Geneva, Dept Cell Biol, Geneva, Switzerland

来源：

FASEB JOURNAL | 2010年 / 24卷 / 10期

关键词：

anticancer drugs; ncRNA; neuroblastoma differentiation; RNA polymerase III; POLYMERASE-III; STEM-CELLS; GROWTH; CANCER; PROLIFERATION; TRANSCRIPTION; PROGNOSIS; RECEPTOR; GENE;

D O I：

10.1096/fj.10-157032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that the synthesis of a pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes, and a dramatic reduction of the tumor initiating cell (TIC) fraction in the NB cell population. Notably, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Altogether, these results suggest the induction of NDM29 expression as possible treatment to increase cancer cells vulnerability to therapeutics and the measure of its synthesis in NB explants as prognostic factor of this cancer type.-Castelnuovo, M., Massone, S., Tasso, R., Fiorino, G., Gatti, M., Robello, M., Gatta, E., Berger, A., Strub, K., Florio, T., Dieci, G., Cancedda, R., Pagano, A. An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells. FASEB J. 24, 4033-4046 (2010). www.fasebj.org

引用

页码：4033 / 4046

页数：14

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