Analysis of human and animal fecal microbiota for microbial source tracking

被引：79

作者：

Lee, Jung Eun ^{[1
]}

Lee, Sunghee ^{[1
]}

Sung, Joohon ^{[2
]}

Ko, GwangPyo ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Environm Hlth, Inst Hlth & Environm, Sch Publ Hlth, Seoul 151742, South Korea

[2] Seoul Natl Univ, Sch Publ Hlth, Dept Epidemiol, Seoul 151742, South Korea

来源：

ISME JOURNAL | 2011年 / 5卷 / 02期

关键词：

gut microbiota; microbial source tracking; 16S rRNA; 454; pyrosequencing; water pollution; GUT MICROBES; CONTAMINATION; SAMPLES; WATER; GENE; PCR;

D O I：

10.1038/ismej.2010.120

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Microbial compositions of human and animal feces from South Korea were analyzed and characterized. In total, 38 fecal samples (14 healthy adult humans, 6 chickens, 6 cows, 6 pigs and 6 geese) were analyzed by 454 pyrosequencing of the V2 region of the 16S rRNA gene. Four major phyla, Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes, were identified in the samples. Principal coordinate analysis suggested that microbiota from the same host species generally clustered, with the exception of those from humans, which exhibited sample-specific compositions. A network-based analysis revealed that several operational taxonomic units (OTUs), such as Lactobacillus sp., Clostridium sp. and Prevotella sp., were commonly identified in all fecal sources. Other OTUs were present only in fecal samples from a single organism. For example, Yania sp. and Bifidobacterium sp. were identified specifically in chicken and human fecal samples, respectively. These specific OTUs or their respective biological markers could be useful for identifying the sources of fecal contamination in water by microbial source tracking. The ISME Journal (2011) 5, 362-365; doi:10.1038/ismej.2010.120; published online 5 August 2010

引用

页码：362 / 365

页数：4

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