Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

被引:48
作者
Wagenaar, JA
van Bergen, MAP
Newell, DG
Grogono-Thomas, R
Duim, B
机构
[1] Inst Anim Sci & Hlth, NL-8200 AB Lelystad, Netherlands
[2] Vet Lab Agcy Weybridge, New Haw, England
关键词
D O I
10.1128/JCM.39.6.2283-2286.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.
引用
收藏
页码:2283 / 2286
页数:4
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