Homogeneous time resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology

被引：102

作者：

Bazin, H ^{[1
]}

Préaudat, M ^{[1
]}

Trinquet, E ^{[1
]}

Mathis, G ^{[1
]}

机构：

[1] CIS Bio Int, F-30204 Bagnols Sur Ceze, France

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2001年 / 57卷 / 11期

关键词：

rare earth cryptates; time resolved fluorescence; fluorescence resonance energy transfer; molecular interactions;

D O I：

10.1016/S1386-1425(01)00493-0

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A homogeneous assay technology using time resolved fluorescence and fluorescence resonance energy transfer is described. A new class of fluorescent complexes, the cryptates, have been used as fluorescent donor with cross-linked allophycocyanin as acceptor. This new donor/acceptor shows an exceptionally high Forster distance R-0 of 9 nm. This allows to build up a set of strategies to probe the interactions of biomolecules in biology, particularly for high throughput screening applications. In this article, we describe the basics of the technology and review applications developed for studying different key molecular interactions involved in cellular processes. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：2197 / 2211

页数：15

共 89 条

[61] FLUORESCENT PHYCOBILIPROTEIN CONJUGATES FOR ANALYSES OF CELLS AND MOLECULES [J].