S-carboxymethylcysteine normalises airway responsiveness in sensitised and challenged mice

被引:23
作者
Takeda, K
Miyahara, N
Kodama, T
Taube, C
Balhorn, A
Dakhama, A
Kitamura, K
Hirano, A
Tanimoto, M
Gelfand, EW
机构
[1] Natl Jewish Med & Res Ctr, Dept Paediat, Denver, CO USA
[2] Okayama Univ, Sch Med, Okayama 700, Japan
关键词
airway hyperresponsiveness; eosinophils; neutrophils; S-carboxymethylcysteine;
D O I
10.1183/09031936.05.00090304
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
S-carboxymethylcysteine (S-CMC) has been used as a mucoregulator in respiratory diseases. However, the mechanism of action of S-CMC on allergic airway inflammation has not yet been defined. In the present study, BALB/c mice were initially sensitised and challenged to ovalbumin (OVA) and, weeks later, re-challenged with OVA (secondary challenge). S-CMC (5-100 mg.kg(-1)) was administered from 2 days before the secondary challenge through to the day of assay. Mice developed airway hyperresponsiveness (AHR) 6 h after the secondary challenge and increased numbers of neutrophils were present in the bronchoalveolar lavage (BAL) fluid. At 72 h after secondary challenge, mice again developed AHR, but the BAL fluid contained large numbers of eosinophils. S-CMC treatment was found to reduce AHR and neutrophilia at 6 h, as well as eosinophilia and AHR at 72 h. These effects appeared to be dose dependent. Goblet cell hyperplasia, observed at 72 h, was reduced by S-CMC. In BAL fluid, increased levels of interferon-gamma, interleukin (IL)-12 and IL-10 and decreased levels of IL-5 and IL-13 were detected. In conclusion, the data indicate that S-carboxymethylcysteine is effective in reducing airway hyperresponsiveness and airway inflammation at two distinct phases of the response to the secondary allergen challenge in sensitised mice.
引用
收藏
页码:577 / 585
页数:9
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