Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

被引：18

作者：

Utsugi, S ^{[1
]}

Sakamoto, W ^{[1
]}

Ogura, Y ^{[1
]}

Murata, M ^{[1
]}

Motoyoshi, F ^{[1
]}

机构：

[1] OKAYAMA UNIV,BIORESOURCES RES INST,KURASHIKI,OKAYAMA 710,JAPAN

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 32卷 / 04期

关键词：

Arabidopsis thaliana; differential screening; flower; transmembrane protein; vegetative storage protein (VSP);

D O I：

10.1007/BF00020217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

引用

页码：759 / 765

页数：7

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