Characterization of a nuclear protein conferring brefeldin A resistance in Schizosaccharomyces pombe

被引:21
作者
Turi, TG
Mueller, UW
Sazer, S
Rose, JK
机构
[1] YALE UNIV, SCH MED, DEPT CELL BIOL, NEW HAVEN, CT 06510 USA
[2] YALE UNIV, SCH MED, DEPT PATHOL, NEW HAVEN, CT 06510 USA
[3] BAYLOR COLL MED, VERNA & MARRS MCLEAN DEPT BIOCHEM, HOUSTON, TX 77030 USA
关键词
D O I
10.1074/jbc.271.15.9166
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The fungal metabolite brefeldin A disrupts protein secretion and causes the redistribution of the Golgi complex 60 the endoplasmic reticulum. Previously we isolated six genes that, when present in multiple copies, confer brefeldin A resistance 60 wild type Schizosaccharomyces pombe. Here we describe the characterization of one of these genes, hba1. This gene encodes an essential protein that shares homology with the mammalian protein RanBP1 and the protein encoded by the Saccharomyces cerevisiae gene YRB1 and contains a peptide motif present in several proteins found within the nuclear pore complex. The protein encoded by hba1 is localized to the nucleus, and it was determined that this protein is phosphorylated in vivo. The characterization of hba1 thus demonstrates a novel mechanism of drug resistance in S. pombe.
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页码:9166 / 9171
页数:6
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