Effect of static pressure on intracellular pH of adhesive Chinese hamster ovary cells

被引：9

作者：

Fujiwara, Masashi ^{[1
]}

Koizumi, Satoru ^{[1
]}

Takagi, Mutsurni ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Kita Ku, Div Biotechnol & Macromol Chem, Sapporo, Hokkaido 0608628, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2007年 / 104卷 / 06期

关键词：

static pressure; intracellular pH; Chinese hamster ovary cells;

D O I：

10.1263/jbb.104.510

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of static pressure on the intracellular pH of the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. In cultivation of CHO cells at 0.9 MPa, two distinct populations were observed in the histogram of a flow cytometer, while single population was observed in cultivation at 0.1 MPa. The intracellular pH of the major population at 0.9 MPa was markedly lower than that of the single population at 0.1 MPa.

引用

页码：510 / 512

页数：3

共 12 条

[1] Vacuolar acidification in Saccharomyces cerevisiae induced by elevated hydrostatic pressure is transient and is mediated by vacuolar H+-ATPase [J].