Ten polymorphic microsatellites from freshwater pearl mussel, Hyriopsis cumingii

被引：23

作者：

Li, Jiale ^{[1
]}

Wang, Guiling

Bai, Zhiyi

Yue, Hua

机构：

[1] Shanghai Fisheries Univ, Shanghai Univ E Inst, Aquaculture Div, 334 Jungong Rd, Shanghai 200090, Peoples R China

[2] Natl Univ Singapore, Temasek Life Sci Lab, Mol & Populat Genet Grp, Singapore 117604, Singapore

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 06期

关键词：

conservation; microsatellites; mussel; pearl;

D O I：

10.1111/j.1471-8286.2007.01880.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Freshwater pearl mussel, Hyriopsis cumingii, is the most economically important mussel species in China. Due to overexploitation and changes of water quality, H. cumingii is facing serious population decline and local extinction. We have isolated and characterized 10 polymorphic microsatellite loci from a genomic DNA library enriched for CA and AG repeats with the aim of developing a set of codominant DNA markers for analysing genetic diversity and population structure of this species. The average allele number of the 10 markers was 7.8 per locus, ranging from two to 16 in 24 unrelated individuals. Nine of 10 markers conformed to Hardy-Weinberg equilibrium and inherited independently, suggesting these microsatellites could be useful for studying population genetics, designing conservation strategies and developing breeding programs.

引用

页码：1357 / 1359

页数：3

共 7 条

[1]

[Anonymous], 2001, Anal Biochem

[2]

Microsatellite enrichment in organisms with large genomes (Allium cepa L.) [J].